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URL: http://www.addgene.org/72639
Proper Citation: RRID:Addgene_72639
Insert Name: UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Comments: PLEASE NOTE- this plasmid has a Stop codon after mCherry which is an error and will cause the construct to not be specifically targeted to the synapse. The Bonkowsky Lab has a corrected plasmid available which will soon be available at Addgene: pTol2-ziUAS:GPHN.FingR-mKate2-ZFI (IL2RGTC)-KRAB(A). Details of cloning are in: Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
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No rating or validation information has been found for pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A).
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Source: Addgene
