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URL: http://www.addgene.org/73148
Proper Citation: RRID:Addgene_73148
Insert Name: sHRPb-NLG
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27240195
Vector Backbone Description: Backbone Size:8451; Vector Backbone:FSW lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R. The plasmid contains the following features (including promoter region): PacI-SynapsinP-XbaI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-AscI Human synapsin promoter 5 aa linker: GSGSG V5 epitope tag: GKPIPNPLLGLDST Lentiviral vector. Derived from FCGW with human synapsin1 promoter replacing CMV
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Source: Addgene
