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Plasmid Name
eSrtA(4S-9) pET29b
RRID:Addgene_75146 RRID Copied  
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RRID:Addgene_75146
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Plasmid Information

URL: http://www.addgene.org/75146

Proper Citation: RRID:Addgene_75146

Insert Name: reprogrammed sortase A variant

Organism: Staphylococcus aureus

Bacterial Resistance: Kanamycin

Defining Citation: PMID:25187567

Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Comments: eSrtA(4S-9) has 120 fold change in substrate specificity with activity comparable to eSrtA and it prefers LPXSG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).

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Ratings and Alerts

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Data and Source Information

Source: Addgene