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URL: http://www.addgene.org/83338
Proper Citation: RRID:Addgene_83338
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:11536; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: VanGlow-GL-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a GFP::Luciferase(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase, and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
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Source: Addgene
