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URL: http://www.addgene.org/83961
Proper Citation: RRID:Addgene_83961
Insert Name: ERT2-spCas9-ERT2
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Sytem Biosciences; Vector Backbone:Piggybac Transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that mutation R1058W in Cas9 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function. The plasmid expresses a ERT2-SpCas9-ERT2 tamoxifen inducible Cas9 driven by the EF-1a promoter. T2A linked puromucin enables stable selection of the piggybac transposon. CRISPR gRNAS can be cloned into the vector at the BbsI cloning sites.
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Source: Addgene
