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Plasmid Name
LeGO-EBFP2
RRID:Addgene_85213 RRID Copied  
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RRID:Addgene_85213
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Plasmid Information

URL: http://www.addgene.org/85213

Proper Citation: RRID:Addgene_85213

Insert Name: EBFP2

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:28109958

Vector Backbone Description: Backbone Size:6417; Vector Backbone:LeGO vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin

Comments: HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de The BsrGI site of EBFP2 can be used to fuse an antibiotic resistance to the fluorescent protein following the building block principle of the LeGO vector system. More information is available on the LeGO website and in the following publications. More references: Weber et al., Molecular Therapy, 2008: http://www.nature.com/mt/journal/v16/n4/full/mt20086a.html Weber et al., Gene Therapy, 2010: http://www.nature.com/gt/journal/v17/n4/full/gt2009149a.html

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Data and Source Information

Source: Addgene