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URL: http://www.addgene.org/85710
Proper Citation: RRID:Addgene_85710
Bacterial Resistance: Chloramphenicol and Kanamycin
Defining Citation: PMID:10888635
Vector Backbone Description: Vector Backbone:pK18; Vector Types:Bacterial Expression, Cre/Lox, Herpesvirus Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: he BAC vector is derived from the E.coli F plasmid. The BAC vector was cloned into the high copy vector pK18, to increse yields. The BAC vector is typically excised by PacI. The BAC vector contains genes for participation (sopA, B,C, a repE gene (required for replication) and an origin of replication (oriS). There is also a chloramphenicol resistance gene. The BAC vector carries a gpt gene, driven by the HSV tk promoter; the gpt gene is followed by the SV40 polyA signal sequence (ca 125 bp). The BAC vector is terminally flanked by loxP sites. The BAC vector seqeunces also carry the EGFP gene, under control of the HCMV MIEP, followed by SV40 polyA signal sequence. The plasmid contains also a 1.5 kb EcoRI-fragment from the murine herpesvirus MHV68. The sequence of pK18 is available from Genbank ACCESSION M17626.
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Source: Addgene
