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URL: http://www.addgene.org/85758
Proper Citation: RRID:Addgene_85758
Insert Name: human-codon-optimized SpCas9
Organism: Streptococcus pyogenes
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:27856772
Vector Backbone Description: Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: Right border (RB) may be truncated in the T-DNA integration. This may result in sgRNA scaffold truncation because the RB is close to 3' end of sgRNA. However, since we efficiently obtained knockout mutants by this vector, the truncation is not serious problem. To overcome it, we also deposited pKI1.1R. In pKI1.1R, sgRNA is not close to RB.
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Source: Addgene
