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URL: http://www.addgene.org/85808
Proper Citation: RRID:Addgene_85808
Insert Name: human-codon-optimized SpCas9
Organism: Streptococcus pyogenes
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:27856772
Vector Backbone Description: Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: Although this vector does not has a problem of sgRNA truncation like pKIR1.1, cloning efficiency in vector construction is low. E. coli with high competency is required.
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Source: Addgene
