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URL: http://www.addgene.org/86264
Proper Citation: RRID:Addgene_86264
Insert Name: ZNF644 homology arms
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:26355004
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: This plasmid was co-transfected along with PX458_ZNF644_1 and PX458_ZNF644_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF644. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
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Source: Addgene
