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URL: http://www.addgene.org/86962
Proper Citation: RRID:Addgene_86962
Insert Name: S.aureus SrtA 5M
Organism: Staphylococcus aureus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28088451
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Pentamutant sortase A was originally described by David R. Liu's laboratory (Harvard University) in: A General Strategy for the Evolution of Bond-Forming Enzymes Using Yeast Display. Chen I, Dorr BM, Liu DR. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404 (PubMed ID: 21697512). As noted in this publication, the cloned sortase A gene lacks the N-terminal 59 amino acids, which do not impact catalytic activity, but these amino acids are still included in the numbering for the mutations.
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Source: Addgene
