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Plasmid Name
pDW363
RRID:Addgene_8842 RRID Copied  
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RRID:Addgene_8842
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Plasmid Information

URL: http://www.addgene.org/8842

Proper Citation: RRID:Addgene_8842

Bacterial Resistance: Ampicillin

Defining Citation: PMID:8635750

Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: This plasmid expression vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused directly to its N-terminus, or, alternatively, as a fusion to the C-terminus of E. coli maltose-binding protein with the BAP on its N-terminus. pDW363 also overproduces biotin holoenzyme synthetase (BirA) to ensure efficient biotinylation of BAP-tagged proteins in vivo. Add biotin to the medium (50 mM) when cultivating cells that contain pDW363 or it derivatives.

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Data and Source Information

Source: Addgene