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URL: http://www.addgene.org/8923
Proper Citation: RRID:Addgene_8923
Insert Name: Clb2-deltaNLS-GFP
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11171327
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS1304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Similar to pPS2189, but with deletion of NLS. Created by PCR amplifying the fragments of CLB2 ORF upstream (0.5kb) and downstream (0.9kb) of the bipartite NLS and joining them using KpnI ends. The KpnI site introduces a glycine and a threonine in place of the bipartite NLS. 2 micron plasmid.
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Source: Addgene
