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URL: http://www.addgene.org/92361
Proper Citation: RRID:Addgene_92361
Insert Name: dCas9-KRAB
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29386245
Vector Backbone Description: Backbone Size:4332; Vector Backbone:x330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. 10.1038/nbt.2675 PubMed 23907171 Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718 Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. 10.1038/nmeth.3630 PubMed 26501517 Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
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Source: Addgene
