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URL: http://www.addgene.org/92394
Proper Citation: RRID:Addgene_92394
Insert Name: chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
Organism: Gallus gallus
Bacterial Resistance: Tetracycline
Defining Citation: PMID:29386245
Vector Backbone Description: Backbone Size:3055; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
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Source: Addgene