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URL: http://www.addgene.org/97012
Proper Citation: RRID:Addgene_97012
Bacterial Resistance: Kanamycin
Defining Citation: PMID:35086530
Vector Backbone Description: Backbone Size:10620; Vector Backbone:p15a ori vector; Vector Types:Mammalian Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Comments: This plasmid is for ES cell targeting of an intersectional Cre/FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, LoxP-flanked EcoRV cloning site and stop cassette, PmeI cloning site, WPRE and bgh pA elements, and a 3' homology arm. A subtractive cDNA of interest (expressed in response to FLP but not Cre recombination) can be cloned into the EcoRV site. The intersectional cDNA of interest (expressed in response to both Cre and FLP recombination) can be cloned into the PmeI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.
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No rating or validation information has been found for p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA.
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Source: Addgene
