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URL: http://www.addgene.org/98700
Proper Citation: RRID:Addgene_98700
Insert Name: Cas9
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29703785
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
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Source: Addgene
