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URL: http://www.addgene.org/99204
Proper Citation: RRID:Addgene_99204
Insert Name: EGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Mathupala, S.P.; Backbone Size:6059; Vector Backbone:pLXIN2; Vector Types:Mammalian Expression, Retroviral, Bicistronic retroviral expression vector; Bacterial Resistance:Ampicillin
Comments: When transfected into amphotropic (PA317) or ecotropic (PE501) mammalian packaging cell lines, the plasmid pLXIN2 will generate live retrovirus. Thus, the procedures will require handling at BSL2 level, when the plasmid is used to generate recombinant retrovirus for transduction. Please note the Kozak sequence is missing a nucleotide; however, this has no functional consequence for expression.
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Source: Addgene
