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URL: http://www.addgene.org/99374
Proper Citation: RRID:Addgene_99374
Insert Name: S. pyogenes sgRNA cassette
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28757163
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014 mTagBFP2 sequence is from Addgene plasmid #34632 (generously shared by Vladislav Verkhusha Lab). "An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. ", Subach et al.. 2014
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Source: Addgene
