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URL: http://www.addgene.org/99377
Proper Citation: RRID:Addgene_99377
Insert Name: S. pyogenes sgRNA cassette
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28757163
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014 iRFP670 sequence is codon-optimized for human (its original sequence is from Addgene plasmid #45453; generously shared by Vladislav Verkhusha Lab). "Near-infrared fluorescent proteins for multicolor in vivo imaging.", Shcherbakova et al.. 2013
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Source: Addgene
