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URL: http://www.addgene.org/103064
Proper Citation: RRID:Addgene_103064
Insert Name: colicin E1 immunity protein
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27795317
Vector Backbone Description: Backbone Marker:Novagen/EMD Millipore; Backbone Size:5227; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This protein was never expressed independently. It was cloned in order to subsequently clone a colicin gene, which would be lethal in the absence of the immunity protein. The cloned fragment was from base pair 500 to base pair 48 of ColE1, which encompasses the E1 immunity protein promoter and the gene.
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Source: Addgene
