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URL: http://www.addgene.org/10679
Proper Citation: RRID:Addgene_10679
Insert Name: PP2A B55 alpha shRNA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14998489
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Comments: To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.
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No rating or validation information has been found for pMKO.1 GFP B55 alpha shRNA 3-1.
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Source: Addgene
