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URL: http://www.addgene.org/107734
Proper Citation: RRID:Addgene_107734
Insert Name: X-3 site gRNA expression cassettes
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30199094
Vector Backbone Description: Backbone Size:5856; Vector Backbone:pYES2; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: pYES2-gRNA-hyg-MCS is an E. coli-S. cerevisiae shuttle plasmid which harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site gRNA (HXK1 gene). However, it can function as an empty gRNA expression backbone plasmid because the existing gRNA expression cassette can be easily replaced with conventional restriction enzyme digestion-ligation method. Moreover, multiple gRNA expression cassettes can be loaded in the MCS.
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Source: Addgene
