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Plasmid Name
pX330.puro sgRNA KGA Stop1
RRID:Addgene_110405 RRID Copied  
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RRID:Addgene_110405
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Plasmid Information

URL: http://www.addgene.org/110405

Proper Citation: RRID:Addgene_110405

Insert Name: GLS glutaminase

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Marker:#110403; Backbone Size:9906; Vector Backbone:pX330.puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin

Comments: sgRNA for GLS exon 19 (KGA isoform stop codon), intended to perform C-Terminal knock-in using pX330 with puromycin resistance. System options: sgRNA: pX330.puro sgRNA KGA Stop1 (Addgene #110405) Homology arms: pUC19 KGA HA5'-mKO2-3' (Addgene #110406) pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407) pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)

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Data and Source Information

Source: Addgene