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URL: http://www.addgene.org/111305
Proper Citation: RRID:Addgene_111305
Insert Name: PRP8
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8725222
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid pJU225 (PRP8 TRPl2p) was constructed from a previously described plasmid JDYl3 (GAL::PRP8) (BROWN and BEGGS 1992). The galactose driven promoter in this plasmid was removed by cutting with NheI and XhoI. A wild-type copy of the promoter was amplified by PCR under nonmutagenic conditions and used to replace the galactose driven promoter. The entire PRP8 gene was then excised using XhoI and NotI and ligated to plasmid pRS424 (TRPl 2p) also cut with XhoI and NotI.
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Source: Addgene
