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URL: http://www.addgene.org/11345
Proper Citation: RRID:Addgene_11345
Insert Name: gateway cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Vector Backbone Description: Backbone Marker:see map of pLexA in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid DNA-binding domain vector. Use this vector for Gateway cloning of a cDNA to be used as the bait in a yeast two-hybrid screen. This vector was modified from plasmid pLexA by placing a Gateway acceptor cassette in the SmaI site of pLexA. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. Gateway recombinational cassettes should be added to primers as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation). Please note that the "author's map" is the map of the original pLexA vector upon which pLexAgtwy is derived - it is not a map of pLexAgtwy. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
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Source: Addgene
