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URL: http://www.addgene.org/117379
Proper Citation: RRID:Addgene_117379
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:31740773
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6332; Vector Backbone:T-Vector pMD19 (Simple); Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This plasmid is designed for transient transfection of protoplasts. It has only been validated in Zea mays mesophyll protoplasts. The region containing the ccdB suicide gene is dropped out by a double-digest with SacI and KpnI and the large fragment is recombined with the DNA insert library.
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Source: Addgene