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URL: http://www.addgene.org/11918
Proper Citation: RRID:Addgene_11918
Insert Name: cre
Organism: bacteriophage P1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10334853
Vector Backbone Description: Backbone Size:6600; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pBS513 carries the wt cre gene under the control of the elongation factor-1 alpha (EF-1 alpha) promoter. The construct carries an upstream Kozak modification to increase expression. The resulting construct provides very strong expression in many different types of cells, including murine embryonic stem (ES) cells. For cell culture work this construct is recommended as a replacement for the CMV-cre construct pBS185 as it provides much stronger expression. However, pBS513 is not suitable for use in transgenic mice as such EF-1 alpha transgene constructs are often inactive in many tissues. Please note: The following enzymes are NOT single cutters: PstI, AflIII, and SacI. We do not suggest using these enzymes for cloning.
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Source: Addgene
