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URL: http://www.addgene.org/11925
Proper Citation: RRID:Addgene_11925
Insert Name: STOP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8231893
Vector Backbone Description: Backbone Size:3806; Vector Backbone:n/a; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pBS302 carries two directly repeated loxP sites flanking a synthetic DNA sequence designated "STOP." The lox-square STOP cassette sits on a NotI fragment that can excised and gel-purified for injection into fertilized zygotes. The STOP sequence is designed to thwart productive expression of a downstream gene under the control of an upstream promoter (to be inserted in the SfiI-SpeI polylinker region). It will have been removed in cells expressing Cre, or in descendants of cells that previously had expressed Cre, because of Cre-mediated recombination at the loxP sites. The STOP sequence is the same as used to regulate T-Ag expression in pBS241.
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Source: Addgene