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URL: http://www.addgene.org/12136

Proper Citation: RRID:Addgene_12136

Insert Name: p53

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:1761576

Vector Backbone Description: Backbone Size:5010; Vector Backbone:pLCR0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: This plasmid, containing the wild type murine p53 cDNA under the control of the previously described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985), was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was digested with EcoRI and HindIII, and the 5010-base pair fragment containing the LCR-SV40 hybrid promoter was isolated and filled in with Klenow. After the ligation of BamHI linkers to the ends of the fragment, regenerating the terminal EcoRI site, the fragment was digested with BamHI and ligated to the 2334-base pair BamHI fragment from the p53 cDNA expression vector pll-4 (Tan et al., 1986). This BamHI fragment contained the entire wild type murine p53 coding region, 50 base pairs of 5'-untranslated sequence, 287 base pairs of 3'-untranslated sequence, and a 3'-located SV40 mRNA splice site and polyA signal.

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