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URL: http://www.addgene.org/12212
Proper Citation: RRID:Addgene_12212
Insert Name: Pak1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11719502
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Addgene has found that this plasmid is extremely slow growing. We recommend keeping plates and liquid cultures growing for at least 20 hours. Overnight will not be sufficient. There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way. Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.
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No rating or validation information has been found for pCMV6M-Pak1 L107F.
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Source: Addgene
