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Plasmid Name
Tmem98-sGFP2
RRID:Addgene_124503 RRID Copied  
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RRID:Addgene_124503
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Plasmid Information

URL: http://www.addgene.org/124503

Proper Citation: RRID:Addgene_124503

Insert Name: Tmem98

Organism: Mus musculus

Bacterial Resistance: Kanamycin

Defining Citation: PMID:31961879

Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector). To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint Please note: The plasmid contains P13S, H81Y, and I229T mutations in SGFP2. These mutations are not known to affect plasmid function.

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Data and Source Information

Source: Addgene