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Plasmid Name
p260 pCMV-CRE-M-AR(wt)
RRID:Addgene_12494 RRID Copied  
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RRID:Addgene_12494
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Plasmid Information

URL: http://www.addgene.org/12494

Proper Citation: RRID:Addgene_12494

Insert Name: CRE-M AR

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:12888538

Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin

Comments: To generate a fusion gene consisting of cre recombinase and the AR LBD, plasmid p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into pCMV-CRE-M(BglII) by digesting two separate aliquots of pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The ligation of these three fragments resulted in the generation of pCMV-CRE-M-AR(wt).

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Data and Source Information

Source: Addgene