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URL: http://www.addgene.org/127519
Proper Citation: RRID:Addgene_127519
Insert Name: Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32444777
Vector Backbone Description: Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note: In 2987 plasmid position, in the UbC promoter, there is a SNP where a G was changed for a T. The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The UbC promoter and _-globin poly(A) signal sequences are derived from pUB-GFP plasmid (Addgene #11155). The original Bxb1 coding sequence (before H. sapiens codon optimization) was obtained from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529324.1).
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Source: Addgene
