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URL: http://www.addgene.org/127762
Proper Citation: RRID:Addgene_127762
Insert Name: Empty backbone
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested with EcoRI to remove the T2A-Puro cassette, and a P2A-Neomycin cassette was amplified by PCR, digested with EcoRI, and ligated.
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Source: Addgene
