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URL: http://www.addgene.org/134310
Proper Citation: RRID:Addgene_134310
Insert Name: kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:31672972
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
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Source: Addgene
