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Plasmid Name
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
RRID:Addgene_136382 RRID Copied  
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RRID:Addgene_136382
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Plasmid Information

URL: http://www.addgene.org/136382

Proper Citation: RRID:Addgene_136382

Insert Name: AaCas12b (D570A)-TV-T2A-MCP-VPR

Organism: Alicyclobacillus acidiphilus

Bacterial Resistance: Spectinomycin

Defining Citation: PMID:32170285

Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin

Comments: TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312

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Data and Source Information

Source: Addgene