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URL: http://www.addgene.org/140205
Proper Citation: RRID:Addgene_140205
Insert Name: mCherry
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32526136
Vector Backbone Description: Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:CRISPR, Synthetic Biology, Proof-of-concept test target of a in filamentous fungi.; Bacterial Resistance:Ampicillin
Comments: To be cotransformed in strains expressing dSpCas9-VPR. This proof-of-concept vector is intended to serve as a positive control and aid the implementation of the CRISPRa vector set in other fungal species, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy when Cas9 is expressed from pCRI006. The mCherry mutation G174D does not appear to affect fluorescence.
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No rating or validation information has been found for pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT.
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Source: Addgene
