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URL: http://www.addgene.org/14444
Proper Citation: RRID:Addgene_14444
Insert Name: Brd4 (888-1362)
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15109495
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 / V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Fragments of human Brd4 cDNA were cloned into pcDNA3.1/V5-His using PCR and TOPO TA Expression Kit (Invitrogen) for expression controlled by both a CMV promoter and a T7 promoter. These plasmids were used for in vitro transcription and translation.
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Source: Addgene