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URL: http://www.addgene.org/14571
Proper Citation: RRID:Addgene_14571
Insert Name: PIK3CA E545K
Organism: Bos taurus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16322248
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
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Source: Addgene
