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URL: http://www.addgene.org/14948
Proper Citation: RRID:Addgene_14948
Insert Name: Fcgamma RIIa
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17110435
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The FcgRIIa cDNA was amplified by RT-PCR using a Titan One Tube RT-PCR System kit (Roche, Mannheim, Germany) and a pair of primers (5'-TTGAATTCACCATGGAGACCCAAATGTCTC-3' and 5'-GCGGCCGCTTAGTTATTACTGTTGACATGG-3'). The PCR product was cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA) to generate pAC01 and subcloned as an EcoRI–NotI fragment into pIRESneo2 (Clontech, Mountain View, CA) to generate pCMV-FcgRIIa-IRES-Neo.
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Source: Addgene
