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URL: http://www.addgene.org/15160
Proper Citation: RRID:Addgene_15160
Insert Name: PLAP
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8455633
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP (A); Vector Types:Retroviral, Avian expression; Bacterial Resistance:Ampicillin
Comments: In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(A) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').
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No rating or validation information has been found for RCASBP-AP (A) (CC#37).
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Source: Addgene
