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Plasmid Name
DAP (DOL-AP) (CC#44)
RRID:Addgene_15164 RRID Copied  
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RRID:Addgene_15164
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Plasmid Information

URL: http://www.addgene.org/15164

Proper Citation: RRID:Addgene_15164

Insert Name: PLAP

Organism: Homo sapiens

Bacterial Resistance: Kanamycin

Defining Citation: PMID:1731342

Vector Backbone Description: Backbone Size:0; Vector Backbone:pDOL; Vector Types:Mammalian Expression, Retroviral, Avian expression; Bacterial Resistance:Kanamycin

Comments: Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter. Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.

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Ratings and Alerts

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Data and Source Information

Source: Addgene