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URL: http://www.addgene.org/155342
Proper Citation: RRID:Addgene_155342
Insert Name: humanized EBpaRS/BstYam
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:32929862
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
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Source: Addgene