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URL: http://www.addgene.org/159747
Proper Citation: RRID:Addgene_159747
Insert Name: gRNA scaffold
Organism: Synthetic
Bacterial Resistance: Kanamycin
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modified from the pHEE401E T-DNA vector described by Wang &al. (2015) Genome Biol 16: 144, doi:10.1186/s13059-015-0715-0: the Hygromycin selection maker of the T-DNA was exchanged with a cassette enabling selection on the basis of CFP-fluorescence in seeds; the egg-cell specific promoter of Cas9 was exchanged with a ubiquitin promoter. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253203v1 for BioRxiv preprint
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Source: Addgene
