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URL: http://www.addgene.org/160230
Proper Citation: RRID:Addgene_160230
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Dianne Duncan; Backbone Size:1961; Vector Backbone:pXZ13lacZalpha; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modification of pXZ13lacZalpha construct to exchange Kanamycin gene for original Ampicillin gene. This improves upon the cloning efficiency of the original pXZ13 vector (reference below) in three ways: 1) The distance between the Esp3I sites is increased to increase cutting efficiency, 2) lacZalpha complementation is introduced to allow for blue/white screening of recombinants, and 3) Kanamycin selection removes 3 of the 4 original parental plasmids from forming colonies in the final plating. Original vector reference: Zhang X, Koolhaas WH, Schnorrer F. A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15;4(12):2409-18
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Source: Addgene
