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Plasmid Name
pCAS-mak10 gRNA-MAK10Δ
RRID:Addgene_160384 RRID Copied  
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RRID:Addgene_160384
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Plasmid Information

URL: http://www.addgene.org/160384

Proper Citation: RRID:Addgene_160384

Insert Name: MAK10

Organism: Saccharomyces cerevisiae

Bacterial Resistance: Kanamycin

Vector Backbone Description: Backbone Size:8743; Vector Backbone:pCAS-mak10 gRNA; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin

Comments: MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains

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Data and Source Information

Source: Addgene