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URL: http://www.addgene.org/16132
Proper Citation: RRID:Addgene_16132
Insert Name: Formylglycine-generating enzyme
Organism: Mycobacterium tuberculosis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17450134
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4081; Vector Backbone:pBAD/myc-his A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The gene encoding Mycobacterium tuberculosis FGE (Rv0712, encoding residues 2-299, GenBank accession NP_215226) was amplified from M. tuberculosis H37Rv genomic DNA and ligated into pBAD/myc-his A between the NcoI and XhoI restriction sites. The FGE gene was placed in frame with the start codon using QuikChange mutagenesis. Please note that this information page previously listed the NcoI cloning site as not destroyed. The site was destroyed during cloning, however, and the page was updated on 6/16/08.
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Source: Addgene
