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URL: http://www.addgene.org/16399
Proper Citation: RRID:Addgene_16399
Insert Name: AdEasier-1 bacterial cells
Organism: E. coli
Bacterial Resistance: Ampicillin and Streptomycin
Defining Citation: PMID:9482916
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin and Streptomycin
Comments: AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system. AdEasier is a trademark of the Johns Hopkins University.
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Source: Addgene