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URL: http://www.addgene.org/165513
Proper Citation: RRID:Addgene_165513
Insert Name: Sulfolobus DNA Polymerase IV
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions; can be used for DNA repair. Poorly processive and error-prone. Involved in untargeted mutagenesis; copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
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Source: Addgene
